Thanks for your post. I hope others may see how many things didn't work out for you in your attempts to handle the parasite by alternative methods. There are just three ways to cure Marine Ich and none include 'reef-safe' medications. You'll find a lot of answers to your questions in these two posts:
Marine Ich - Myths and Facts
and
http://www.reefland.com/forum/marine...e-process.html
The only advice I will give and provide is what is in the best interests of the fish. So you won't find much sympathy for the work you have ahead.
1. What's most important is to get the fish cured as soon as possible. Move the fish to the quarantine tank immediately. You will watch for ammonia and nitrite signs. If any show up, make large water changes to control these until the biological filter takes hold. This may mean 2 or 3 water changes per day; removing all uneaten food; removing all visual signs of waste; and keeping the tank very clean.
2. In this case, since the fish are presumably only infected with one parasite, putting them together is okay for the treatment.
3. Leave the display go fallow for 8 weeks, no less. Read the above posts.
4. There should be no substrate in the hospital/quarantine tank. Using some of the filter material in the outside filter is a great idea. But, no substrate. Regarding your concerns about ammonia and nitrite, see 1. above.
5. The Ammonia alert tabs were not made for saltwater and they work very poorly for this. Do not use dip sticks, either. Perform no less than 3 tests with a test kit for both ammonia and nitrite every day. Test about 1-2 hours after a feeding and before lights out. There should be none detectable in order to skip a large water change.
6. I would have thought that after all the chemical dosing you've done that you would have come to the conclusion that additives are not very good for the tank, the fish, nor their condition. Whether you treat with copper or use the hyposalinity method, do not dose with chemicals. Chemicals may interact with the medication or the treatment method. The sheet you cut that absorbs ammonia is worthwhile to continue to use as well as the product Algone. These are absorbents and don't put things into the water. Stick with chemical absorbents rather than additives. ;;
Read through those two posts very closely, please. If you have further questions, don't hesitate to ask. We're here to help!



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