I agree that live rock can aid in denitrification. That's why you want to have some bigger rocks instead of a ton of smaller ones. With the larger rocks deep in the center there will be a larger anoxic zone so denitrification can take place.
But as far as proving wether a sand bed can aid in denitrification, couldn't someone with access to the cheap Southdown sand do a simple test?
All you probably need is a 10 gallon tank, a powerhead, and a dead shrimp, and a little live sand. You don't even need lighting (just something to control evaporation).
Put a four inch sandbed in the tank and cycle it like you would cycle dead sand normally stirring the sand regularly. After a while the Nitrates should spike, and now if the sandbed is left alone the theory can be tested.
Maybe the same could be done with the Coraline algae idea. Culture the algae on a smooth serface like a sterilized beer bottle or some small tupper wear until it encrusts it completely. Then put it in a small bare tank with detectable nitrates and see if it does anything. You won't need a light in the tank to keep the algae alive just keep it by indirect sunlight. I've seen plenty of Coraline grow in a sump under a tank in front of a window. The only thing that might affect the experiment is if green algae begins to grow.
Any other ideas on that, or has someone already done an experiment similar to this?



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